ELISA Test

**"This product is not distributed in the European Union. It does not bear the CE marking as it has not undergone the conformity assessment procedures of the European Directive 98/79/EC, for in vitro medical devices".

The ELISA is a fundamental tool of clinical immunology, and is used as an initial screen for HIV, HCV and  HBsAG detection. Based on the principle of antibody-antibody interaction, this test allows for easy visualization of results and can be completed without the additional concern of radioactive materials use.

Certificates: GMP, ISO, FSC

What are the advantages of ELISA?
ELISA tests are generally highly sensitive and specific and compare favorably with other methods used to detect substances in the body, such as radioimmune assay (RIA) tests. They have the added advantages of not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus).

ELISA UNITEST

-HIV ELISA Test

An HIV ELISA, sometimes called an HIV enzyme immunoassay (EIA) is the first and most basic test to determine if an individual is positive for a selected pathogen, such as HIV. The test is performed in a 8 cm x 12 cm plastic plate which contains an 8 x 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter.

-HCV ELISA Test (Hepatitis C)

Premier Anti-Hepatitis C Antigen Elisa Test is a third generation qualitative enzyme immunoassay for detection of Anti-Hepatitis C Antigen (Anti-HCV) in human serum.
Premier Anti-HCV ElisaTest is based on the principle of inter-second antibody enzyme immunoassay procedure.

The test is performed in a 8 cm x 12 cm plastic plate which contains an 8 x 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter.

-HBsAG ELISA Test (Hepatitis B)

Premier Anti-Hepatitis B Antigen Elisa Test is a third generation qualitative enzyme immunoassay for detection of Anti-Hepatitis B Antigen (Anti-HBsAG ) in human serum.

The test is performed in a 8 cm x 12 cm plastic plate which contains an 8 x 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter.

	Partially purified, inactivated HIV antigens pre-coated onto an ELISA plate
	Patient serum which contains antibodies. If the patient is HIV+, then this serum will contain antibodies to HIV, and those antibodies will bind to the HIV antigens on the plate.
	Anti-human immunoglobulin coupled to an enzyme. This is the second antibody, and it binds to human antibodies.
	Chromogen or substrate which changes color when cleaved by the enzyme attached to the second antibody.

                    Positive ELISA                            Negative ELISA          

Above is ELISA data from three patients. Numbers are expressed as optical density at 450nm. The cutoff value indicating a positive result is 0.500. Optical densities of 0.300 to 0.499 are indeterminate and need to be retested. Values below 0.300 are considered to be negative. In most cases, a patient will be retested if the serum gives a positive result. If the ELISA retests are positive, the patient will then be retested by western blotting analysis.

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